What is Vitamin A?
Vitamin A is a group of fat-soluble retinoids, including retinol, retinal (retinaldehyde), retinoic acid, and retinyl esters. It is an essential nutrient for humans and plays crucial roles in various physiological processes, most notably vision, immune function, cell growth and differentiation, and epithelial integrity.
Vitamin A exists in two primary forms in the human diet:
- Preformed Vitamin A (Retinol) – found in animal-based foods.
- Provitamin A carotenoids (like beta-carotene) – found in plant-based foods, which the body converts into retinol as needed.
The active form, retinol, is essential for many biological functions and is stored in the liver for later use. Vitamin A is vital from early development through adulthood, making it one of the most important nutrients for overall health.
Table of Contents
🔬 Chemical Structure and Properties of Vitamin A
Vitamin A is a group of fat-soluble retinoids, including retinol, retinal (retinaldehyde), retinoic acid, and retinyl esters. It also includes provitamin A carotenoids like beta-carotene, which are plant-derived precursors that the body can convert into active vitamin A.
🔸 1. Forms of Vitamin A
✅ Preformed Vitamin A (Animal Sources):
These are biologically active forms found in animal products.
- Retinol (C₂₀H₃₀O)
- A primary alcohol with a long isoprenoid side chain
- Most common storage form in the body
- Converts reversibly to retinal
- Retinal (C₂₀H₂₈O)
- Aldehyde form of vitamin A
- Critical for vision (forms part of the light-sensitive retinal pigment, rhodopsin)
- Retinoic Acid (C₂₀H₂₈O₂)
- Acidic form, cannot be converted back to retinal or retinol
- Regulates gene expression and cellular differentiation (especially in skin and immune cells)
- Retinyl Esters
- Storage form in the liver and other tissues
- Synthesized by esterifying retinol with long-chain fatty acids (e.g., palmitate)
✅ Provitamin A (Plant Sources):
- Beta-carotene (C₄₀H₅₆)
- A carotenoid pigment found in carrots, sweet potatoes, etc.
- Cleaved enzymatically in the intestine to yield two molecules of retinal
🧬 Chemical Structure Overview of Vitamin A
Here’s a breakdown of retinol, the base compound of vitamin A:
Molecular Formula: C₂₀H₃₀O
Molecular Weight: ~286.45 g/mol
Functional Groups:
- Alcohol group (–OH) at the terminal end
- Conjugated double bonds along the carbon chain, giving it its yellow/orange color
Structure Type:
- Long isoprenoid chain with alternating double bonds (polyene structure)
- Hydrophobic (non-polar)
🔁 Isomerism:
Vitamin A compounds can exist in cis and trans isomeric forms:
- All-trans-retinol is the most active form in human physiology.
- 11-cis-retinal plays a critical role in vision (in rhodopsin formation in the retina).
| Property | Details |
|---|---|
| Solubility | Fat-soluble (insoluble in water, soluble in fats and oils) |
| Stability | Sensitive to heat, light, and oxygen — easily degraded |
| Color | Yellow to orange pigment (due to conjugated double bonds) |
| Absorption | Absorbed in the small intestine with dietary fats |
| Storage | Mainly stored in the liver as retinyl esters |
| Transport | Carried in blood bound to retinol-binding protein (RBP) and transthyretin |
Because of its fat solubility, vitamin A is stored in the body and can build up to toxic levels if consumed in excess.(alert-warning)
Functions of Vitamin A
Vitamin A is a fat-soluble vitamin that is essential for several biological functions in the human body. It is required for normal vision, growth and development, immune system function, and skin health. This vitamin plays a crucial role in maintaining the health of the eyes, skin, and mucous membranes, and also supports healthy bone growth and reproductive system function.
Vision: Vitamin A is essential for maintaining healthy vision. It plays a crucial role in the functioning of the retina, the part of the eye that is responsible for converting light into nerve signals that the brain can interpret as vision.
Immune system: Vitamin A is essential for maintaining a healthy immune system. It helps to stimulate the production and activity of white blood cells that fight off infections and other foreign invaders.
Skin health: Vitamin A is important for maintaining healthy skin. It helps to regulate the growth and differentiation of skin cells, promoting healthy skin cell turnover and preventing dryness, acne, and other skin problems.
Growth and development: Vitamin A is important for normal growth and development, particularly in children. It helps to support bone growth, promote healthy teeth and gums, and maintain healthy skin and hair.
Reproduction: Vitamin A is important for reproductive health in both men and women. It plays a role in sperm production and fetal development during pregnancy.
Key Forms of Vitamin A and Their Functions
Vitamin A exists in several interconvertible forms within the human body, each serving distinct physiological roles. These include Retinol, Retinal (Retinaldehyde), Retinoic Acid, and Retinyl Esters. Together, these forms are crucial for maintaining vision, cellular health, immunity, and development.
1. Retinol – The Transport and Storage Form
Description: Retinol is often referred to as the alcohol form of vitamin A and is the main circulating form in the blood. It is either ingested directly through animal-based foods or converted from provitamin A carotenoids (like beta-carotene) from plant-based sources.
Function:
- Acts as a transport molecule, delivering vitamin A to tissues where it's needed.
- Can be reversibly converted to retinal or retinyl esters depending on the body's requirements.
- Plays a role in reproduction, immune response, and skin health.
Importance: Since it can be converted into other forms, retinol is central to vitamin A metabolism and regulation.
2. Retinyl Esters – The Storage Form
Description: Retinyl esters are the storage form of vitamin A, primarily stored in the liver. These are formed when excess retinol is esterified (combined with fatty acids) for long-term storage.
Function:
- Serve as a reserve that the body can draw upon when dietary intake is insufficient.
- When needed, retinyl esters are hydrolyzed back into retinol, which is then transported to various tissues.
Importance: Essential for maintaining a steady supply of vitamin A during periods of dietary deficiency or increased physiological demand (e.g., pregnancy, illness).
3. Retinal (Retinaldehyde) – The Vision-Related Form
Description: Retinal is the aldehyde form of vitamin A and plays a critical role in the visual cycle.
Function:
- Combines with the protein opsin to form rhodopsin, the pigment in rod cells of the retina responsible for vision in low-light conditions.
- In response to light, the retina undergoes a structural change (from cis to trans form), triggering a signal that is interpreted by the brain as vision.
Importance: Deficiency in retinal affects the production of rhodopsin, leading to night blindness, one of the earliest signs of vitamin A deficiency.
4. Retinoic Acid – The Gene Regulator and Developmental Agent
Description: Retinoic acid is the oxidized, acidic form of vitamin A that cannot be converted back to retinol or retinal.
Function:
- Binds to nuclear receptors in cells, specifically retinoic acid receptors (RARs) and retinoid X receptors (RXRs), to regulate the expression of genes involved in growth and differentiation.
- Crucial in the development and maintenance of epithelial tissues, which line the skin, lungs, gastrointestinal tract, and other organs.
- Influences immune function, wound healing, and fetal development (especially limb, heart, and eye formation).
Importance: Retinoic acid is often used in dermatology for acne, psoriasis, and skin aging treatments due to its effects on skin cell turnover. It is also used medically to treat certain cancers, like acute promyelocytic leukemia.
Each form of vitamin A serves a distinct yet interconnected role in the body:
- Retinol acts as the main transporter and precursor to other forms.
- Retinyl esters provide storage to ensure long-term availability.
- Retinal is essential for vision, especially under low light.
- Retinoic acid regulates gene expression and cellular development.
Dietary Sources for Vitamin A
There are two main types of vitamin A found in the human diet:
1. Preformed Vitamin A (Retinol and Retinyl Esters)
Found in animal-based foods
- Active form of vitamin A; readily used by the body
2. Provitamin A (Carotenoids, especially Beta-Carotene)
Found in plant-based foods
- Must be converted by the body into active vitamin A
- Acts as an antioxidant and vitamin precursor
🥩 Animal-Based Sources of Vitamin A (Preformed Vitamin A)
These sources provide retinol, which is directly absorbed and used:
🧠 1. Liver (Extremely Rich Source)
Beef liver – 3 oz cooked provides over 6,000 mcg RAE (over 600% of the Daily Value)
Chicken liver, pork liver, cod liver oil are also extremely rich
⚠️ Liver is so rich in vitamin A that excessive intake (especially during pregnancy) can be toxic.
🥚 2. Eggs
Egg yolks – contain modest but useful amounts of vitamin A
🧈 3. Dairy Products
Whole milk, cheese, butter, and cream contain vitamin A
Skim milk is often fortified with vitamin A, since it's lost when the fat is removed
🐟 4. Fish and Fish Oils
Cod liver oil – one of the richest sources
Salmon, mackerel, tuna, and sardines contain moderate amounts
🥕 Plant-Based Sources of Vitamin A (Provitamin A Carotenoids)
These foods are high in beta-carotene, alpha-carotene, and beta-cryptoxanthin, which the body converts into vitamin A:
🥬 1. Dark Green Leafy Vegetables
- Spinach
- Kale
- Collard greens
- Swiss chard
Even though they’re green, they contain high levels of carotenoids that are masked by chlorophyll.
🥕 2. Orange and Yellow Vegetables
- Carrots – 1 medium carrot provides over 500 mcg RAE
- Sweet potatoes – one medium baked sweet potato provides ~1,096 mcg RAE (122% DV)
- Pumpkin
- Butternut squash
- Yellow corn
🍑 3. Orange and Yellow Fruits
- Mangoes
- Cantaloupe (melon)
- Apricots
- Papaya
- Red or pink grapefruit
🌶️ 4. Red and Orange Peppers
Rich in beta-carotene and other carotenoids
🌟 Fun Fact: Brightly colored vegetables are often excellent sources of provitamin A.
🥣 Fortified Foods Containing Vitamin A
In many countries, foods are fortified with vitamin A to prevent deficiency:
- Fortified breakfast cereals
- Fortified margarine
- Fortified skim milk and plant-based milks (soy, oat, almond)
- Meal replacement drinks and infant formulas
💊 Vitamin A Supplements
Vitamin A is available in two primary forms:
1. Preformed Vitamin A (Retinoids)
- Found in animal products and supplements as retinyl palmitate or retinyl acetate
- More readily absorbed and stored in the liver
- Used in higher doses for therapeutic purposes (e.g., treating deficiency or severe acne)
2. Provitamin A (Beta-Carotene)
- Plant-derived form found in colorful vegetables (carrots, sweet potatoes)
- Converted by the body into retinol as needed
- Considered safer in terms of overdose risk
- Available in supplements for general support
⚠️ Always consult a healthcare provider before starting vitamin A supplements, especially during pregnancy or when taking other medications.
⚠️ Safety and Toxicity Concerns of Vitamin A
Vitamin A, especially in preformed form, can be toxic in high doses:
Symptoms of Vitamin A Toxicity (Hypervitaminosis A):
- Nausea, vomiting
- Headache, dizziness
- Blurred vision
- Liver damage
- Bone thinning
- Birth defects (if taken in excess during pregnancy)
Tolerable Upper Intake Level (UL) for adults: 3,000 mcg RAE/day (10,000 IU)
❗ Pregnant women should be especially cautious with supplements, as excessive vitamin A can cause birth defects. Beta-carotene is usually preferred in prenatal multivitamins.
🌱 Natural Alternatives of Vitamin A
If supplementation isn't necessary, you can maintain adequate vitamin A intake by eating:
- Liver, eggs, dairy (preformed vitamin A)
- Carrots, sweet potatoes, spinach, kale, mangoes (beta-carotene)
Vitamin A supplements are helpful in cases of deficiency or specific medical needs, but must be used under medical guidance.
It is important to note that Vitamin A toxicity can occur from excessive consumption of supplements. Therefore, it is recommended to obtain Vitamin A from a balanced diet and not rely on supplements unless advised by a healthcare professional.(alert-warning)
Recommended Daily Intake (RDA) of Vitamin A
| Group | RDA (mcg RAE/day) | Upper Limit (mcg/day) | Notes |
|---|---|---|---|
| Men (age 19+) | 900 mcg RAE | 3,000 mcg | RAE = Retinol Activity Equivalents |
| Women (age 19+) | 700 mcg RAE | 3,000 mcg | Includes pregnant & non-pregnant adults |
| Pregnant Teens (14–18) | 750 mcg RAE | 2,800 mcg | Higher needs for fetal development |
| Pregnant Women (19+) | 770 mcg RAE | 3,000 mcg | Supports placental and fetal growth |
| Lactating Teens (14–18) | 1,200 mcg RAE | 2,800 mcg | To support milk production |
| Lactating Women (19+) | 1,300 mcg RAE | 3,000 mcg | Vitamin A passes into breastmilk |
| Children (1–3 years) | 300 mcg RAE | 600 mcg | Rapid growth stage |
| Children (4–8 years) | 400 mcg RAE | 900 mcg | Supports development |
| Children (9–13 years) | 600 mcg RAE | 1,700 mcg | Early adolescence |
Causes of Vitamin A Deficiency
Vitamin A deficiency occurs when there is insufficient intake of Vitamin A in the diet or inadequate absorption and utilization of Vitamin A from food. Vitamin A is a fat-soluble vitamin and is mainly stored in the liver. The deficiency of Vitamin A can lead to various health problems and diseases.
Here are some common causes of Vitamin A deficiency:
Inadequate intake of Vitamin A
Vitamin A is mainly obtained from animal sources such as liver, eggs, dairy products, and fish. Vegetables and fruits also contain a precursor to Vitamin A called beta-carotene, which can be converted into Vitamin A in the body. A diet lacking in these sources can lead to Vitamin A deficiency.
Malabsorption Disorders
Certain medical conditions, such as Crohn's disease, celiac disease, cystic fibrosis, and pancreatic disorders, can interfere with the absorption and utilization of Vitamin A in the body.
Alcoholism
Chronic alcoholism can lead to the destruction of liver cells, which are responsible for storing Vitamin A. This can result in the depletion of Vitamin A stores in the liver and lead to deficiency.
Chronic diarrhea and intestinal parasites
Chronic diarrhea and intestinal parasites can cause malabsorption of nutrients, including Vitamin A.
Breastfeeding
Infants who are exclusively breastfed by mothers who are deficient in Vitamin A may develop a deficiency, as breast milk may not provide enough Vitamin A to meet the infant's requirements.
Zinc deficiency
Zinc is essential for the conversion of beta-carotene into Vitamin A. Zinc deficiency can lead to a decrease in the conversion of beta-carotene to Vitamin A.
Kidney and Liver Diseases
People with kidney and liver diseases may have impaired conversion and utilization of Vitamin A.
It is important to note that Vitamin A deficiency is more common in developing countries, particularly in regions where malnutrition is prevalent. In such regions, a lack of access to nutrient-dense foods, poverty, and poor sanitation can all contribute to Vitamin A deficiency.(alert-success)
Symptoms of Vitamin A Deficiency
Vitamin A is an essential nutrient for maintaining healthy vision, skin, and immune function. A deficiency in vitamin A can lead to a range of symptoms, some of which can be severe. The symptoms of vitamin A deficiency vary depending on the severity and duration of the deficiency.
1.) Eye Problems such as:
- Xerophthalmia - is a medical condition in which the eye fails to produce tears
- Night blindness - the ability to see at night is affected.
- Keratomalacia - drying or clouding of your corneas,
- Bitot’s spots – white plaques of keratinized epithelial cells – are found on the conjunctiva of young children with vitamin A deficiency.
- Corneal ulcers - Open sores on the cornea.
2. Skin problems: Vitamin A is important for maintaining healthy skin, and a deficiency in this vitamin can cause a range of skin problems, including dryness, itching, and a scaly texture.
3. Respiratory infections: Vitamin A is important for maintaining the health of the respiratory system, and a deficiency in this vitamin can increase the risk of respiratory infections, such as pneumonia and bronchitis.
4. Delayed growth and development: Vitamin A is essential for growth and development, and a deficiency in this vitamin can lead to stunted growth and delayed development in children.
5. Increased susceptibility to infections: Vitamin A is important for maintaining a healthy immune system, and a deficiency in this vitamin can increase the risk of infections and make it more difficult for the body to fight off infections.
Long-Term Complications of Vitamin A Deficiency
Vitamin A deficiency can lead to a variety of long-term complications, especially in developing countries where malnutrition is more prevalent.
Night blindness
One of the early symptoms of Vitamin A deficiency is night blindness, which occurs due to impaired vision in low-light conditions. This is because Vitamin A is required for the synthesis of rhodopsin, a pigment in the eye that enables vision in dim light. Night blindness can significantly impair an individual's quality of life, especially in areas with limited access to electricity or adequate lighting.
Xerophthalmia
Xerophthalmia is a severe eye condition caused by prolonged Vitamin A deficiency. It is characterized by dryness, thickening, and clouding of the cornea, which can lead to permanent vision loss if left untreated. Xerophthalmia is a common cause of blindness in children in developing countries.
Increased Susceptibility to Infections
Vitamin A is essential for maintaining a healthy immune system, and its deficiency can increase an individual's susceptibility to infections, particularly in children. It impairs the body's ability to produce white blood cells that fight off infections, making them more vulnerable to diseases such as measles, pneumonia, and diarrhea.
Growth Retardation
Vitamin A deficiency can significantly impair a child's growth and development, leading to stunted growth, poor bone development, and delayed sexual maturation. It can also cause reduced appetite and weight loss, further exacerbating the problem.
Anemia
Vitamin A deficiency can also contribute to the development of anemia, a condition characterized by low levels of red blood cells in the body. This is because Vitamin A is required for the production of hemoglobin, the protein that carries oxygen in the blood.
Birth Defects
Vitamin A deficiency during pregnancy can increase the risk of birth defects, such as neural tube defects, cleft lip and palate, and heart defects. These complications can be life-threatening and have long-term consequences on the health of the child.
Infertility
Vitamin A deficiency can affect both male and female fertility and may lead to difficulty conceiving or carrying a pregnancy to term.
Vitamin A deficiency can lead to various long-term complications that can significantly affect an individual's health and quality of life.
Diagnosis of Vitamin A Deficiency
A diagnosis of vitamin A deficiency can be made based on a combination of symptoms, physical exams, and laboratory tests.
One of the most common methods of diagnosing vitamin A deficiency is through a physical examination. The doctor will look for symptoms such as dry or scaly skin, night blindness, and other signs of poor vision. They may also ask about the patient's diet and any history of gastrointestinal problems that could affect the absorption of vitamin A.
Blood tests can also be performed to determine the levels of vitamin A in the body. A simple blood test can reveal whether there is a deficiency or not. If the levels are low, additional tests may be necessary to determine the underlying cause of the deficiency. These tests can include measuring levels of other vitamins and minerals, checking for underlying conditions that may affect absorption, and conducting imaging studies to examine the eyes and other tissues.
In addition to blood tests, other diagnostic tests may be used to determine the extent of the deficiency and any underlying causes. These tests can include skin biopsy, eye exams, and imaging studies such as X-rays or CT scans.
It is important to note that not all cases of vitamin A deficiency are the same. The severity of the deficiency can vary from person to person, and the diagnosis and treatment will depend on the specific circumstances of each case. A doctor or healthcare provider will be able to provide the best course of action for managing the deficiency and any underlying conditions that may be contributing to it.
Management of Vitamin A Deficiency
Treatment for vitamin A deficiency depends on the severity of the deficiency and the underlying cause. Mild deficiencies can often be corrected through dietary changes, while more severe deficiencies may require supplementation or other medical interventions.
The primary approach to managing Vitamin A deficiency is through supplementation. Vitamin A supplements come in different forms, including tablets, capsules, and liquid drops. In developing countries, Vitamin A supplements are typically distributed through national health programs, where they are provided for free or at a low cost to individuals who are at risk of deficiency. Supplementation is an effective way to prevent and treat Vitamin A deficiency, and it has been shown to improve health outcomes significantly.
In addition to supplementation, dietary changes can also help manage Vitamin A deficiency. Foods rich in Vitamin A include liver, fish, dairy products, eggs, and fruits and vegetables that are high in carotenoids, such as sweet potatoes, carrots, and spinach. Encouraging individuals to consume a varied and balanced diet that includes these foods can help prevent Vitamin A deficiency.
For individuals who have already developed symptoms of Vitamin A deficiency, high-dose Vitamin A supplements may be necessary. These supplements are typically given as a single dose or over a few days, depending on the severity of the deficiency. In severe cases, hospitalization may be required to administer the supplements safely.
In addition to supplementation and dietary changes, community-based programs can also help manage Vitamin A deficiency. For example, educating individuals on the importance of Vitamin A and the foods that contain it can increase awareness and encourage dietary changes. Fortifying staple foods, such as rice or sugar, with Vitamin A can also help prevent deficiency.
It's important to note that vitamin A supplementation should only be taken under the guidance of a healthcare provider, as excessive intake can lead to toxicity. In addition, it's important to address any underlying medical conditions or lifestyle factors that may contribute to vitamin A deficiency (such as malabsorption, alcoholism, or poor dietary habits) to prevent future deficiencies.
Prevention of Vitamin A Deficiency
The most effective way to prevent Vitamin A deficiency is through adequate dietary intake. Vitamin A is found in a variety of animal and plant-based foods. The most common and richest sources of Vitamin A are liver, eggs, milk, cheese, butter, oily fish, and fortified foods such as breakfast cereals, margarine, and some types of bread.
Plant-based foods such as fruits and vegetables also contain provitamin A carotenoids, which are converted into Vitamin A in the body. Some of the best sources of provitamin A carotenoids include carrots, sweet potatoes, dark leafy greens like spinach, kale, and collard greens, and colorful fruits like mangoes, papayas, and cantaloupes.
In addition to dietary sources, supplements can also be used to prevent Vitamin A deficiency. Vitamin A supplements are available in both synthetic and natural forms. However, it is important to note that excessive supplementation can lead to toxicity and should be avoided.
Another way to prevent Vitamin A deficiency is through breastfeeding. Breast milk is an excellent source of Vitamin A, and exclusively breastfeeding infants for the first six months of life can help prevent deficiency.
Food fortification is also an effective way to prevent Vitamin A deficiency. In many countries, staple foods such as sugar, oil, and flour are fortified with Vitamin A to increase their availability and accessibility.
Lastly, addressing underlying health conditions such as chronic diarrhea and malabsorption disorders can also help prevent Vitamin A deficiency. These conditions can impair the body's ability to absorb nutrients, including Vitamin A.
Vitamin A Toxicity - Hypervitaminosis A
Vitamin A toxicity, also known as hypervitaminosis A, occurs when there is an excessive accumulation of vitamin A in the body. This can occur either through overconsumption of vitamin A-rich foods or high-dose supplementation.
Symptoms of Vitamin A toxicity
Symptoms of vitamin A toxicity can range from mild to severe and may include:
- Nausea and vomiting
- Headache
- Dizziness
- Blurred vision
- Dry, itchy skin
- Bone pain and fractures
- Joint pain and stiffness
- Hair loss
- Fatigue
- Liver damage (in severe cases)
In pregnant women, excessive intake of vitamin A can cause birth defects and fetal abnormalities.
In severe cases, vitamin A toxicity can lead to coma and death.
Diagnosis of Vitamin A toxicity
A doctor can diagnose vitamin A toxicity by reviewing the patient's medical history, conducting a physical examination, and performing blood tests to measure the levels of vitamin A in the body. In some cases, imaging tests may be necessary to assess liver damage.
Management of Vitamin A toxicity
The treatment for vitamin A toxicity depends on the severity of the symptoms. In mild cases, stopping the intake of vitamin A supplements and foods high in vitamin A can resolve the symptoms. However, in severe cases, hospitalization may be necessary to manage symptoms and provide supportive care.
Prevention of Vitamin A toxicity
Preventing vitamin A toxicity involves being mindful of vitamin A intake, especially when taking supplements or consuming foods high in vitamin A. The recommended daily intake for vitamin A is 900 micrograms for adult men and 700 micrograms for adult women. It is important to not exceed this recommended intake and to speak with a healthcare provider before taking vitamin A supplements.
It's important to note that this includes both preformed vitamin A (found in animal-based foods) and beta-carotene (found in plant-based foods). Excessive consumption of vitamin A supplements, particularly in the form of retinol, can increase the risk of toxicity. Chronic consumption of high levels of beta-carotene from plant-based sources is unlikely to cause toxicity, as the body converts beta-carotene to vitamin A as needed and eliminates any excess.
If you suspect that you may be experiencing symptoms of vitamin A toxicity, it's important to talk to your healthcare provider. They may recommend reducing vitamin A intake or monitoring for potential complications. It's also important to avoid taking high-dose vitamin A supplements without the guidance of a healthcare provider.
Vitamin A, especially in its active form, retinol, is essential for maintaining healthy vision, strong immunity, reproductive health, and skin integrity. A well-balanced diet that includes both animal-based and plant-based sources can help ensure an adequate intake. While deficiency can lead to serious health consequences, excessive supplementation should be avoided to prevent toxicity. Maintaining the right balance is key to reaping the full benefits of this powerful vitamin.(alert-success)
